Volume 3,Issue 7
丹酚酸A抗炎症作用的机制研究
目的:通过网络药理学和分子对接技术,探讨丹酚酸A(Salvianolic acid A,SalA)抗炎作用的靶点及其作用机制。方法:以多个数据库预测SalA的作用靶点以及炎症相关的靶点,并取交集,得到SalA治疗炎症的关键靶点;用STRING数据库进行分析,并进行基因本体论(Gene Ontology,GO)功能及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。通过AutoDock Vina进行分子对接模拟验证。结果:共获得SalA作用预测靶点578个、炎症靶点2022个,药物、疾病共同交集的靶点有140个,暗示SalA可能通过凋亡通路、焦亡通路等发挥抗炎作用。分子对接验证核心靶点与SalA之间亲和力较强。结论:SalA可能通过多个炎症蛋白结合,多靶点调控多通路发挥抗炎作用。
[1]国家卫生健康委员会. 2023中国卫生健康统计年鉴 [M]. 北京:中国协和医科大学出版社. 2024.
[2]Xie Y, Xu E, Bowe B, et al. Long-term cardiovascular outcomes of COVID-19 [J]. Nature Medicine. 2022, 28(3):583-590.
[3]Chew DS, Li Y, Cowper PA, et al. Cost-effectiveness of catheter ablation versus antiarrhythmic drug therapy in atrial fibrillation: the CABANA randomized clinical trial [J].
Circulation. 2022, 146(7):535-547.
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临床研究 | CLINICAL RESEARCH
[4]Fu M, Zhang F, Song Y, et al. Salvianolic acid A alleviates sciatic nerve injury in rats via inhibiting NLRP3 inflammasome through the activation of Schwann cells
Autophagy [J]. Neurochemical Research. 2025, 50(3):152.
[5]Zhai Q, Shang S, Zhang Z, et al. Mechanism of salvianolic phenolic acids and hawthorn triterpenic acids combination in intervening atherosclerosis: network pharmacology,
molecular docking, and experimental validation [J]. Frontiers in Pharmacology. 2025, 16:1501846.
[6]Li J, Wang N, Huang Q, et al. Acute Treatment with salvianolic acid A produces neuroprotection in stroke models by inducing excitatory long-term synaptic depression [J].
Acs Chemical Neuroscience. 2025, 16(4):659-672.
[7]Qian S, Chen G, Li R, et al. Disulfide stress and its role in cardiovascular diseases [J]. Redox Biology. 2024, 75:103297.
[8]Jiang Y, Cai Y, Han R, et al. Salvianolic acids and its potential for cardio-protection against myocardial ischemic reperfusion injury in diabetes [J]. Front Endocrinol (Lausanne).
2024, 14:1322474.
[9]Li Y, Tu Z, Chen F, et al. Anti-inflammatory effect of Danhong injection through inhibition of GSDMD-mediated pyroptosis [J]. Phytomedicine. 2023, 113:154743.
[10]Liao HJ, Tzen JTC. The Potential Role of Phenolic Acids from Salvia miltiorrhiza and Cynara scolymus and Their Derivatives as JAK Inhibitors: An In Silico Study [J].
International Journal of Molecular Sciences. 2022, 23(7):4033.
[11]Shi J, Zhao Y, Wang K, et al. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death [J]. Nature, 2015, 535(7610):153-8.
[12]Ho JH, Hong CY. Salvianolic acids: small compounds with multiple mechanisms for cardiovascular protection [J]. Journal of Biomedical Science. 2011, 18(1):30.
[13]Chen X, Liu G, Yuan Y, et al. NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-κB signaling [J]. Cell Death & Disease. 2019,
10(12):906.
[14]Sarapultsev A, Gusev E, Komelkova M, et al. JAK-STAT signaling in inflammation and stress-related diseases: implications for therapeutic interventions [J]. Molecular
Biomedicine. 2023, 4(1):40.
[15]Ma Q, Yang Q, Chen J, et al. Salvianolic Acid A Ameliorates Early-Stage Atherosclerosis Development by Inhibiting NLRP3 Inflammasome Activation in Zucker Diabetic
Fatty Rats [J]. Molecules. 2020, 25(5):1089.
[16]Dong S, Liang S, Cheng Z, et al. ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in
colorectal cancer [J]. Journal of Experimental & Clinical Cancer Research. 2022, 41(1):15.
[17]Sun EJ, Wankell M, Palamuthusingam P, et al. Targeting the PI3K/Akt/mTOR Pathway in Hepatocellular Carcinoma [J]. Biomedicines. 2021, 9(11):1639.
[18]Diab SE, Tayea NA, Elwakil BH, et al. In vitro and in vivo anti-colorectal cancer effect of the newly synthesized sericin/propolis/fluorouracil nanoplatform through
modulation of PI3K/AKT/mTOR pathway [J]. Scientific Reports. 2024, 14(1):2433.
[19]Qian W, Wang Z, Xu T, et al. Anti-apoptotic effects and mechanisms of salvianolic acid A on cardiomyocytes in ischemia-reperfusion injury [J]. Histology And
Histopathology. 2019, 34(3):223-231.
[20]Wang R, Song F, Li S, et al. Salvianolic acid A attenuates CCl4-induced liver fibrosis by regulating the PI3K/AKT/mTOR, Bcl-2/Bax and caspase-3/cleaved caspase-3
signaling pathways [J]. Drug Design Development And Therapy. 2019, 13:1889-1900.