Volume 1,Issue 3
Analysis of the Improving Effect of Shenlian Granules on Rats with Chronic Atrophic Gastritis Based on Antioxidant and Liver and Kidney Protection Mechanisms
Objective: To investigate the ameliorative effects of Shenlian Granules on rats with chronic atrophic gastritis from the perspectives of antioxidant and liver and kidney protection. Methods: A rat model of chronic atrophic gastritis was established using three combined methods，and then selected the best modeling method for this experiment. That is, chemical method (alternating free access to 0.1% ammonia solution and 20 mmol/L sodium deoxycholate solution) + hunger and satiety method (alternating one day of satiety followed by one day of hunger) + physical stimulation (30-minute tail clamping with a plastic clip on the satiety day). The model was maintained for 10 consecutive weeks. After confirming successful modeling by observing changes in the intrinsic glands of the gastric mucosa of SD rats, the rats were randomly divided into six groups: a model group, a control group, and groups receiving Shenlian Granules at low, medium, and high concentrations. Treatment and observation began at week 11. The treatment groups (low, medium, and high concentration groups) were gavaged with Shenlian Granules (1.65 g/kg, 3.3 g/kg, and 6.6 g/kg, respectively). The control group was gavaged with Morodan (2.43 g/kg). The model and blank groups were gavaged with purified water (2 mL per rat) once daily for 10 weeks. During modeling and treatment, the rats’ general condition was observed, and post-treatment weight changes were recorded. Sixteen hours after the last gavage, blood was collected from the abdominal aorta under anesthesia. Serum ALT, AST, BUN, and Cre levels were measured by an automated biochemical analyzer, and serum MDA, T-SOD, and GSH levels were measured by ELISA. Results: Shenlian Granules effectively improved the mental activity and body weight of rats with chronic atrophic gastritis. Shenlian Granules reduced serum ALT, AST, BUN, Cre and MDA levels, and increased T-SOD and GSH-Px levels in rats with chronic atrophic gastritis (p < 0.01). The differences were statistically significant and concentration-dependent. Conclusion: Shenlian granules may restore chronic atrophic gastritis by enhancing antioxidant capacity. It also plays a protective role in liver and kidney function during the drug action process, which further provides a reliable experimental basis for the clinical use of this prescription in the treatment of patients with chronic atrophic gastritis.
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